Phage DNA Kit

Product Overview:

The Phage DNA Isolation Kit provides a fast and efficient method for the isolation and purification of total DNA from bacteriophages propagated in liquid bacterial cultures. The procedure avoids phenol, chloroform, and cesium chloride, offering a safe and reliable alternative for phage DNA extraction.

Designed for small volumes of phage supernatant (as little as 1 mL), the spin column workflow can be completed in under 45 minutes. The purified DNA is of high integrity and suitable for a wide range of molecular biology applications, including Southern blotting, restriction fragment length polymorphism (RFLP), sequencing, cloning, and real-time PCR.

Purification Technology:

Purification is based on spin column chromatography, which selectively binds nucleic acids while removing proteins and contaminants. The workflow includes:

1. Starting from clarified phage supernatant (free of bacterial debris)
2. Lysis of phage particles using heat and chemical treatment
3. Addition of isopropanol and loading of lysate onto a spin column
4. Selective DNA binding under ionic conditions
5. Removal of proteins, RNA, and impurities via wash steps
6. Elution of purified phage DNA ready for downstream use

Key Features:

• Optimized for DNA extraction from bacteriophages grown in liquid cultures
• No phenol, chloroform, or cesium chloride required
• Efficient processing of 1 mL phage supernatant
• Rapid workflow – <45 minutes per preparation
• Produces high-quality, PCR-ready DNA

Applications:

• Southern blot analysis
• Restriction Fragment Length Polymorphism (RFLP)
• Sequencing and cloning
• qPCR and real-time PCR
• General molecular biology and phage research