AmpTec Amplification Technologies

ExpressArt FFPE mRNA Amplification kit Key Features

• Uniform and complete amplification of mRNA fragments with and without poly(A)(low 3‘-5‘-ratios in the range of 1 to 3)
• High presence calls for clinical FFPE samples
• microarray platforms with a single probe per gene:
  up to 100% of parallel cryo samples e.g. with Novartis NovaChips
• microarray platfors with multiple probes per gene:
  up to 50% of parallel cryo samples e.g. Affymetrix GeneChips (probe sets) "X3P GeneChips" the special adaptation to FFPE samples is not required.
• High concordance for differentially expressed genes
• qualitative in Decrease or Increase
• quantitative in Fold Changes

» FFPE-Presentation (PDF-file, 131 KB)

NucleoGuard (NG) is a small molecular weight, isotrophic reagent that acts as a mimic for nucleic acids and is useful in the isolation of RNA from very small samples (<10 ng RNA) and/or difficult samples, with special benefits for FFPE samples.

• Its high concentration leads to effective inhibition of all nucleic acid recognition reactions, including the activities of RNases, nucleases, etc., and of polymerases.
• Its effective removal with standard spin columns results in no interference with down-stream applications, e.g. reverse transcription
• For FFPE samples, its high concentration leads to (partial) disruption of nucleic acid aggregates and improved RNA recovery (see also DeCrossLinker)

DeCrossLinker (DCL) is a small molecular weight reagent for the (partial) reversion of cross-links in FFPE samples, like RNA-protein, RNA-RNA and RNA-DNA cross-links.
Its use leads to

• increased liberation of RNAs
• reduced stop sites for reverse transcriptase
• (partial) disruption of nucleic acid aggregates, which is especially effective in combination with NucleoGuard

This results in more efficient template RNAs for reverse transcriptase, as evident in reduced PCR cycle numbers (4 to 7) in quantitative RT-PCRs.